Publications by Year: Submitted

Ishigaki K, Lagattuta K, Luo Y, James E, Buckner J, Raychaudhuri S. HLA autoimmune risk alleles restrict the hypervariable region of T cell receptors [Internet]. Submitted; medRxivAbstract
Polymorphisms in the human leukocyte antigen (HLA) genes within the major histocompatibility complex (MHC) locus strongly influence autoimmune disease risk15. Two non-exclusive hypotheses exist about the pathogenic role of HLAalleles; i) the central hypothesis, where HLA risk alleles influence thymic selection so that the probability of T cell receptors (TCRs) reactive to pathogenic antigens is increased68; and ii) the peripheral hypothesis, where HLA risk alleles increase the affinity for pathogenic antigens911. The peripheral hypothesis has been the main research focus in autoimmunity, while human data on the central hypothesis are lacking. Here, we investigated the influence of HLA alleles on TCR composition at the highly diverse complementarity determining region 3 (CDR3), where TCR recognizes antigens. We demonstrated unexpectedly powerful HLA-CDR3 associations. The strongest association was found at HLA-DRB1 amino acid position 13 (n = 628 subjects, explained variance = 9.4%; P = 4.1 x 10−138). This HLA position mediates genetic risk for multiple autoimmune diseases. In structural analysis of TCR-peptide-MHC complexes, we observed that HLA-DRB1 position 13 does not interact directly with CDR3, but is proximate to antigenic peptide residues that are also close to CDR3. We identified multiple CDR3 amino acid features enriched by HLA risk alleles; for example, the risk alleles of rheumatoid arthritis, type 1 diabetes, and celiac disease all increase the hydrophobicity of CDR3 position 109 (P < 2.1 x 10−5). In the setting of celiac disease, the CDR3 features favored by HLA risk alleles are more enriched among candidate pathogenic TCRs than control TCRs (P = 2.4 × 10−6 for gliadin specific TCRs). Together, these results provide novel genetic evidence supporting the central hypothesis.